The cellular abundance of transcription factors (TFs) is an important determinant of their regulatory activities. Deriving TF copy numbers is therefore crucial to understanding how these proteins ...

Mitochondria carry out numerous metabolic reactions that are critical to cellular homeostasis. Here we present a protocol for interrogating mitochondrial metabolites and measuring their matrix ...

In a recent medRxiv* preprint manuscript, researchers from Singapore and the United States report the development of a digital CRISPR method for the sensitive, specific, and rapid detection of viral ...

Technical processes like thin-film deposition and etching, and surface modification extensively use plasma discharges. Hence, it is important to quantitatively characterize plasma sources for ...

QPrESTs consists of 50-150 amino acids identical to a part of the corresponding human protein. They are produced recombinantly in the presence of stable isotope-labeled (i.e. heavy) amino acids to ...

Absolute Quantification (AQUA) is an innovative and powerful targeted quantitative proteomics technique that has gained widespread popularity across various proteomics studies. This cutting-edge ...

Researchers from Critical Analytics for Manufacturing Personalized-Medicine (CAMP), an Interdisciplinary Research Group (IRG) at the Singapore-MIT Alliance for Research and Technology (SMART), MIT's ...

Digital PCR (dPCR) is a powerful technology for detecting and quantifying nucleic acids, providing unparalleled precision and sensitivity. This innovative technology is particularly effective at ...

Whitehead Institute scientists have developed a method to quickly isolate and systematically measure metabolite concentrations within the cellular organelles known as mitochondria, often referred to ...

Mass spectrometry just isn't inherently quantitative because of differences within the ionization efficiency and/or detectability of the many peptides in a given sample, which has sparked the ...

Scientists have developed a method to quickly isolate mitochondria from mammalian cells and systematically measure the concentrations of mitochondrial metabolites. Mitochondrial dysfunction is found ...