How To Perform a Bacterial Transformation
7:02
YouTubeBio-Rad Explorer
How To Perform a Bacterial Transformation
This video demonstrates how to transform bacteria using the Bio-Rad Explorer #pGLO Bacterial Transformation Kit. The CaCl2 method is used to transform E. coli HB101 bacteria. Proper aseptic technique, heat-shock method, and sample labeling are shown. This technique may also be used with the Bio-Rad Explorer pGLO Bacterial Transformation and ...
37.6K viewsSep 11, 2019
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Tomasz Grzywa on Instagram: "From plasmid to engineered cells 🧬🦠 Step 1 - Cell seeding HEK293T cells are seeded the day before transfection to reach optimal confluency, ensuring high viability and maximal transfection efficiency. What are HEK293T cells and why use them? HEK293T cells are a human embryonic kidney-derived cell line engineered to express SV40 large T antigen, which enhances plasmid replication and expression. They are highly transfectable, robust in culture, and support efficient
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